Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
217700 | Journal of the Chinese Institute of Chemical Engineers | 2007 | 7 Pages |
One of the difficulties encountered in the control of bioprocesses is the lack of reliable on-line sensors for key state variables. On-line measurements of the recombinant biomass and intracellular protein are the key requirements to achieving an effective process operation. This paper investigates the suitability of using on-line estimation to predict concentrations of biomass and protein for recombinant Escherichia coli cultivated in batch and fed-batch modes. Various pairs of on-line accessible measurements of glucose, lactate, and acetate are fed back into the on-line estimator to train the tuning parameters in order to predict concentrations of biomass and intracellular protein. Such predictions are consistent with experimental data using off-line measurements.