A Dynamic Complex of Signaling Proteins Uses Polar Localization to Regulate Cell-Fate Asymmetry in Caulobacter crescentus

SummaryCellular asymmetry is critical to metazoan development and the life cycle of many microbes. In Caulobacter, cell cycle progression and the formation of asymmetric daughter cells depend on the polarly-localized histidine kinase CckA. How CckA is regulated and why activity depends on localization are unknown. Here, we demonstrate that the unorthodox kinase DivL promotes CckA activity and that the phosphorylated regulator DivK inhibits CckA by binding to DivL. Early in the cell cycle, CckA is activated by the dephosphorylation of DivK throughout the cell. However, in later stages, when phosphorylated DivK levels are high, CckA activation relies on polar localization with a DivK phosphatase. Localization thus creates a protected zone for CckA within the cell, without the use of membrane-enclosed compartments. Our results reveal the mechanisms by which CckA is regulated in a cell-type-dependent manner. More generally, our findings reveal how cells exploit subcellular localization to orchestrate sophisticated regulatory processes.

Graphical AbstractFigure optionsDownload full-size imageDownload high-quality image (291 K)Download as PowerPoint slideHighlights► DivL, an unorthodox kinase, promotes activation of the master kinase CckA ► Phosphorylated DivK binds directly to DivL to block the activation of CckA ► Cell cycle and cellular asymmetry are driven by changes in DivK phosphorylation ► Full activation of CckA depends on polar localization with a DivK phosphatase, PleC