Synthesis of 3D hierarchical porous Co3O4 film by eggshell membrane for non-enzymatic glucose detection

•The 3D hierarchical porous Co3O4 film was prepared by employing eggshell membrane as bio-templates.•The 3D hierarchical film is more effective in facilitating reactants diffusion and the electron transfer.•The Co3O4-based sensor was applied for direct electrochemical non-enzymatic detection of glucose.•It shows high sensitivity (366.03 μAmM− 1 cm− 2), fast response (3 s), excellent stability and anti-interference performance.

We provide a facile, cost-effective and environmentally friendly method to prepare 3D hierarchical porous Co3O4 film by employing eggshell membrane as bio-templates. The Co3O4 nanofibers interconnected with each other to construct a 3D hierarchical porous film with macropores and mesopores, which is more effective in facilitating reactants diffusion and the electron transfer. The developed sensor was applied for direct electrochemical non-enzymatic detection of glucose. High sensitivity (366.03 μA mM− 1 cm− 2), fast response (3 s), anti-interference performance and excellent stability were achieved. The results demonstrate that the 3D hierarchical porous Co3O4 film have great potential applications in developing direct electrochemical non-enzymatic glucose biosensors.

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