Mitochondrial recombination in natural populations of the button mushroom Agaricus bisporus

In the majority of sexual eukaryotes, the mitochondrial genomes are inherited uniparentally and have predominantly clonal population structures. In clonally evolving genomes, alleles at different loci will be in significant linkage disequilibrium. In this study, the associations among alleles at nine mitochondrial loci were analyzed for 379 isolates in four natural populations of the button mushroom Agaricus bisporus. The results indicated that the mitochondrial genome in the Desert California population was not significantly different from random recombination. In contrast, the three other populations all showed predominantly clonal mitochondrial population structure. While no evidence of recombination was found in the Alberta, Canada A. bisporus population, signatures of recombination were evident in the Coastal Californian and the French populations. We discuss the potential mechanisms that could have contributed to the observed mitochondrial recombination and to the differences in allelic associations among the geographic populations in this economically important mushroom.

► Examined associations among alleles at nine mitochondrial loci in four populations. ► Found no evidence of mitochondrial recombination for the Alberta, Canada population. ► Evidence for both clonality and recombination in Coastal Californian and French populations. ► Mitochondria in the desert Californian population recombined randomly. ► Discussed potential mechanisms for the differences among the four populations.