Article ID Journal Published Year Pages File Type
218131 Journal of Electroanalytical Chemistry 2015 5 Pages PDF
Abstract

•A novel competitive PEC aptasensor for thrombin detection is proposed.•The detection is based on the TiO2 electrode coupled with TB–AuNPs–GOx labels.•The aptasensor exhibits wide linear range and low detection limit.•The aptasensor was successfully applied for the detection of TB in human plasma.

A novel competitive photoelectrochemical aptasensor for thrombin (TB) detection is proposed using TiO2 electrode coupled with TB-Au nanoparticles (AuNPs)–glucose oxidase (GOx) labels. To construct the aptasensor, chitosan was coated on the TiO2 surface for the further immobilization of TB aptamer that was bridged by the use of glutaraldehyde. TB detection was then performed based upon the competitive interaction of TB and TB-AuNPs-GOx bioconjugate with TB aptamer on the electrode. GOx could catalyze the glucose to produce H2O2, which acts as an effective electron donor to deplete the photogenerated holes located at the valence band of excited TiO2, resulting in the enhanced photocurrent response. Under the optimal conditions, the as-prepared PEC aptasensor exhibits excellent analytical property for detection of TB in the range from 1.0 × 10− 12 mol L− 1 to 1.0 × 10− 8 mol L− 1 with a detection limit of 1.9 × 10− 13 mol L− 1. Moreover, the aptasensor was also successfully applied for the determination of TB in human plasma. The present strategy provides a promising method for the future development of PEC aptasensor for advanced biochemical analysis.

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Physical Sciences and Engineering Chemical Engineering Chemical Engineering (General)
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