Article ID Journal Published Year Pages File Type
2181711 Fungal Genetics and Biology 2007 9 Pages PDF
Abstract

The asexual ascomycete Aspergillus oryzae has an steA homolog in its genome. The steA homolog of A. oryzae was transcribed in the wild-type strain but steA disruption did not affect the phenotype of the disruptant strain. On the other hand, the steA-overexpressing strain showed the restriction of vegetative hyphal growth and conidiation, and the formation of balloon-shaped structures. The restriction of vegetative hyphal growth and conidiation were partially rescued by high osmolarity. In addition, the hyphal growth of the steA-overexpressing strain was inhibited by Calcofluor white, and the strain was found to be sensitive to cell-wall-degrading reagents. Increase of secretory cell-wall-degrading enzymes of the steA-overexpressing strain was detected. These results indicate that SteA regulates cell-wall-degrading enzymes and that the phenotypes of A. oryzae steA-overexpressing strain may be due to cell wall abnormalities. steA may participate in cell wall metabolism.

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