Total antioxidant potential assay with cyclic voltammetry and/or differential pulse voltammetry measurements

In the literature many different TAP assays are described. They use various analytical methods. However, it seems that natural methods of investigation of the redox reactions are electroanalytical techniques. Indeed, TAP has been already estimated potentiometrically and voltammetrically.In the paper we propose new electrochemical assay of TAP measurements. In this case TAP measure is the integral of the product of current and exponent from the potential (related to the standard reduction potential of the hydroxyl radicals) along normal or differential pulse voltammetric peak. It was found that TAP values can be accurately and robustly measured using described assay. They are directly proportional to the sample concentration and dependent on its redox potential. The proposed assay has been tested on pure compounds and on the mixtures. It turned out that in the second case TAP is an additive value for non-interacted compounds. On the other side, it is non-additive for compounds interacting with each other. As the real samples to test elaborated assay the extracts of various herbs are selected. Melissa is characterized by the strongest antioxidative properties.

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