Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2190754 | Journal of Molecular and Cellular Cardiology | 2011 | 13 Pages |
We previously demonstrated that syntaxin (Syn)-1A is present in the sarcolemma of rat cardiomyocytes and binds sulfonylurea receptor (SUR) 2A nucleotide binding folds (NBFs) to inhibit ATP-sensitive potassium (KATP) channel. Here, we examined for the precise domains within the NBFs of SUR2A that may interact with Syn-1A. Specifically, we tested truncated NBF protein segments encompassing the conserved motifs Walker A (WA), signature/Linker (L), and Walker B (WB). In vitro binding results indicate that the domains encompassing WA and L of NBF-1 and all three conserved motifs of NBF-2 bound Syn-1A. Electrophysiological studies, employing inside-out patch-clamp recordings from SUR2A/Kir6.2 expressing HEK cells and mouse cardiomyocytes, show that WB and L of NBF-1 and all three NBF-2 truncated protein segments reduced Syn-1A inhibition of SUR2A/KATP channels. Remarkably, these same NBF-1 and -2 truncated proteins could independently disrupt the intimate FRET interactions of full length SUR2A (− mCherry) and Syn-1A (− EGFP). These results taken together indicate that Syn-1A possibly maintains inhibition of cardiac ventricular KATP channels by binding to large regions of NBF-1 and NBF-2 to stabilize the NBF-1-NBF-2 heterodimer formation and prevent ATP-binding and ATP hydrolysis. Since KATP channels are closely coupled to metabolic states, we postulate that these very intimate Syn-1A-SUR2A interactions are critically important for myocardial protection during stress, in which profound changes in metabolic factors (pH, ATP) could modulate these Syn-1A-SUR2A interactions.
► Syn-1A binds and inhibits SUR2A/KATP channels through NBF-1-L and -WB. ► Syn-1A binds and inhibits SUR2A/KATP channels through all conserved motifs of NBF-2. ► Syntaxin-1A could potentially act as a scaffolding protein to regulate cardiac KATP channels.