Increased phospholamban phosphorylation limits the force–frequency response in the MLP–/– mouse with heart failure

Reduced Ca2+ release from the sarcoplasmic reticulum (SR) and a negative force–frequency relation characterize end-stage human heart failure. The MLP–/– mouse with dilated cardiomyopathy is used as a model to explore novel therapeutic interventions but the alterations in Ca2+ handling in MLP–/– remain incompletely understood. We studied [Ca2+]i in left ventricular myocytes from MLP–/– and WT mice (3–4 months old; whole-cell voltage clamp, 30 °C). At 1 Hz stimulation, the amplitude of [Ca2+]i transients was similar. However, in contrast to WT, at higher frequencies the [Ca2+]i transient amplitude declined in MLP–/– and there was no increase in SR Ca2+ content. Unexpectedly, the decline of [Ca2+]i was faster in MLP–/– than in WT (at 1 Hz, τ of 80 ± 9 vs. 174 ± 29 ms, P < 0.001) and the frequency-dependent acceleration of the decline was abolished suggesting an enhanced basal SERCA activity. Indeed, the Ca2+ affinity of SR Ca2+ uptake in homogenates was higher in MLP–/–, with the maximal uptake rate similar to WT. Phosphorylation of phospholamban in MLP–/– was increased (2.3-fold at Ser16 and 2.9-fold at the Thr17 site, P < 0.001) with similar SERCA and total phospholamban protein levels. On increasing stimulation frequency to 4 Hz, WT, but not MLP–/–, myocytes had a net gain of Ca2+, suggesting inadequate Ca2+ sequestration in MLP–/–. In conclusion, increased baseline phosphorylation of phospholamban in MLP–/– leads to a reduced reserve for frequency-dependent increase of Ca2+ release. This represents a novel paradigm for altered Ca2+ handling in heart failure, underscoring the importance of phosphorylation pathways.