| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2195622 | Molecular and Cellular Endocrinology | 2016 | 10 Pages |
•MTR-A2756G increases the risk of idiopathic male infertility in Iranian men.•MTR-A2756G is associated with male infertility in Asian and Caucasian populations.•D919G substitution has a significant effect on the function and structure of MTR.
Methionine synthase (MTR) has a crucial role in DNA synthesis and methylation reactions. The aim of this study was to investigate the association of the MTR-A2756G polymorphism with idiopathic male infertility. Blood samples were collected from 217 idiopathic infertile- and 233 healthy-men, and MTR-A2756G genotyping was performed by PCR-RFLP. Meta-analysis was conducted by pooling our data with the data obtained from 6 previous studies. Also, the effects of this substitution on protein structure were evaluated by bioinformatics tools. Our study revealed the association of AG-genotype, GG-genotype, and G-allele with male infertility. Meta-analysis showed a significant association between A2756G transition and male infertility. In addition, structural analysis of the transition effect on protein revealed a significant influence on MTR function (with score: 38; expected accuracy: 66%). These findings suggest that the A2756G substitution might be a genetic risk factor and a potential biomarker for idiopathic male infertility.
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