| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2196251 | Molecular and Cellular Endocrinology | 2012 | 10 Pages |
Subcutaneous (SAT) and visceral adipose tissue (VAT) differ in biochemical and metabolic properties, especially when obesity is present. We submitted paired SAT and VAT samples from six morbidly obese patients and six non-obese persons to two-dimensional differential gel electrophoresis and matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight mass spectrometry. Compared with non-obese subjects, obese patients presented with increased carboxylesterase-1, zinc finger protein 324A, annexin A5, ubiquitin carboxyl-terminal hydrolase, α-crystallin B chain, osteoglycin, retinal dehydrogenase-1 and 14-3-3 protein γ, and decreased transferrin, complement C3, fibrinogen γ chain, albumin, α1-antitrypsin and peroxiredoxin-6, irrespective of the adipose tissue depot studied. SAT and VAT differed in protein species of fibrinogen and osteoglycin, whereas adipose tissue depot and obesity interacted on the protein abundance of actin, α-actinin 1, one protein species of carboxylesterase-1, retinal dehydrogenase-1 and 14-3-3 protein γ. Our nontargeted proteomic approach identified novel protein species that may be involved in the development of obesity in humans.
► Subcutaneous (SAT) and visceral adipose tissue (VAT) differ in biological properties. ► 2D-DIGE and MALDI-TOF/TOF identified differences in protein abundance between SAT and VAT. ► Obesity influenced the abundance of several proteins irrespective of adipose tissue depot. ► Obesity and adipose tissue depot interacted on the abundance of some proteins. ► The proteins identified in this study might contribute to obesity and its complications.
