Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2196911 | Molecular and Cellular Endocrinology | 2010 | 9 Pages |
Abstract
Aim of the present study was to investigate whether estrogens were able to directly activate rapid signaling pathways controlling spermatogenesis in rat pachytene spermatocytes (PS). Classically, estrogens act by binding to estrogen receptors (ERs) α and β. Recently, it has been demonstrated that rapid estrogen action can also be activated through the G-protein-coupled receptor (GPR)-30. Herein, we demonstrated that rat PS express ERα, ERβ and GPR30. Treatment of PS with estradiol (E2), the selective GPR30 agonist G1 and the selective ERα agonist PPT determined activation of ERK1/2 which are part of GPR30 signaling cascade. ERK1/2 activation in response to E2 and G1 was correlated to an increased phosphorylation of c-Jun. All treatments failed to induce these responses in the presence of EGFR inhibitor AG1478, ERK inhibitor PD98059 and ER inhibitor ICI182780. mRNA expression of cell cycle regulators cyclin A1 and B1 was downregulated by E2 and G1 while an up-regulation of proapoptotic factor Bax was observed in the same conditions. These data demonstrate that E2, working through both ERα and/or GPR30, activates in PS the rapid EGFR/ERK/c-Jun pathway, modulating the expression of genes involved in the balance between cellular proliferation and apoptosis.
Keywords
Pachytene spermatocytesGPR30HB-EGFERβERαAP-1ERKOEGFRERSATFGAPDHPPTJnkCREArKOc-Jun N-terminal kinasecAMPERK1/2MAPKMAPK kinaseCyclic adenosine monophosphateEstradiolEstrogenSpermatocytesApoptosisactivating transcription factorMEKRatactivator protein-1mitogen-activated protein kinaseextracellular regulated kinase 1/2glyceraldehyde 3-phosphate dehydrogenaseEstrogen receptor αEstrogen receptor βEpidermal growth factor receptorestrogen receptors
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Authors
Adele Chimento, Rosa Sirianni, Christelle Delalande, Dorothèe Silandre, Camille Bois, Sebastiano Andò, Marcello Maggiolini, Serge Carreau, Vincenzo Pezzi,