Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2197166 | Molecular and Cellular Endocrinology | 2009 | 7 Pages |
Abstract
Growth factors activate ATF2 via sequential phosphorylation of Thr69 and Thr71, where the ATF2-Thr71-phosphorylation precedes the induction of ATF2-Thr69 + 71-phosphorylation. Here, we studied the mechanisms contributing to serum-induced two-step ATF2-phosphorylation in JNK1,2-deficient embryonic fibroblasts. Using anion exchange chromatography, ERK1/2 and p38 were identified as ATF2-kinases in vitro. Inhibitor studies as well as nuclear localization experiments show that the sequential nuclear appearance of ERK1/2 and p38 determines the induction of ATF2-Thr71 and ATF2-Thr69 + 71-phosphorylation in response to serum.
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Authors
Bart Baan, Gerard C.M. van der Zon, J. Antonie Maassen, D. Margriet Ouwens,