Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2201409 | Neurochemistry International | 2009 | 5 Pages |
Abstract
The controversial and limited data on the distribution of dopamine (DA) receptors of type 4 (D4) in the human brain prompted us to explore their density and pharmacological characteristics in the prefrontal cortex, striatum and hippocampus, through a series of binding assays. Brain samples were taken during autopsy from seven subjects. Tissue homogenates were incubated with increasing concentration of [3H]-YM-09151-2, a D2-like receptor antagonist, and L-745,870 and/or sulpiride to define the non-specific binding, while PPAP was used to block sigma receptors. The results showed a low density of D4 receptors in the hippocampus only, with a preponderance of D2/D3 and sigma receptors in the prefrontal cortex and striatum. In conclusion, these findings underline that it is possible to label D4 receptors by means of [3H]-YM-09151-2, provided that D2, D3 and sigma receptors are blocked.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Cell Biology
Authors
Donatella Marazziti, Stefano Baroni, Irene Masala, Gino Giannaccini, Laura Betti, Lionella Palego, Mario Catena Dell'Osso, Giorgio Consoli, Maura Castagna, Antonio Lucacchini,