Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2202254 | Neurochemistry International | 2007 | 5 Pages |
Abstract
To produce antibodies that permit the immunohistochemical discrimination of choline acetyltransferase of the common type (cChAT) from its splice variant of a peripheral type (pChAT), we immunized rabbits with a cChAT specific recombinant protein encoded by ChAT exons 7 and 8 of the rat cChAT gene. Successful antibody production was proved by Western blotting on rat brain and on HEK293 cells expressing green fluorescent protein (GFP), cChAT-GFP and pChAT-GFP. By immunohistochemistry our antiserum clearly labeled known cholinergic structures in rat brain, but gave no positive staining in the trigeminal ganglion which contained many neurons positive with pChAT antiserum.
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Authors
Shin Kimura, Jean-Pierre Bellier, Akinori Matsuo, Ikuo Tooyama, Hiroshi Kimura,