Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2203253 | Seminars in Cell & Developmental Biology | 2007 | 12 Pages |
Abstract
The role of membrane traffic is to transfer cargo between distinct subcellular compartments. Each individual trafficking event involves the creation, transport and fusion of vesicular and tubular carriers that are formed and regulated via cytoplasmic coat protein complexes. The dynamic nature of this process is therefore highly suitable for studying using live cell imaging techniques. Although these approaches have raised further questions for the field, they have also been instrumental in providing essential new information, in particular relating to the morphology of transport carriers and the exchange kinetics of coat proteins and their regulators on membranes. Here, we present an overview of live cell-imaging experiments that have been used in the study of coated-vesicle transport, and provide specific examples of their impact on our understanding of coat function.
Keywords
COPER–Golgi intermediate compartmentTransitional ERSPIMTIRFMER exit siteERGICclathrin-coated pitCLEMCI-MPRGEFTGNCCPBFAYFPEREsFCSCFPGFPCCDFRAPGGAFLIMRNA interferenceRNAicoated vesiclesFluorescence resonance energy transferFRETbrefeldin AMembrane trafficLive cell imagingfluorescence lifetime imagingTERCharge-Coupled DeviceTrans-Golgi networkendoplasmic reticulumGAPfluorescence correlation spectroscopyguanine nucleotide exchange factorfluorescence recovery after photobleachingElectron microscopyTotal internal reflection fluorescence microscopycorrelative light and electron microscopyadaptor proteinGTPase-activating proteinyellow fluorescent proteingreen fluorescent proteinGreen fluorescent protein (GFP)cyan fluorescent proteincoat protein complexcation-independent mannose 6-phosphate receptor
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Authors
Els Wessels, Jeremy C. Simpson,