| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2204876 | Trends in Cell Biology | 2008 | 8 Pages |
Abstract
Chromophore-assisted laser inactivation (CALI) is a technique whereby engineered proteins and dye molecules that produce substantial amounts of reactive oxygen species upon absorption of light are used to perturb biological systems in a spatially and temporally defined manner. CALI is an important complement to conventional genetic and pharmacological manipulations. In this review, we examine the applications of CALI to cell biology and discuss the underlying photochemical mechanisms that mediate this powerful technique.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Cell Biology
Authors
Ken Jacobson, Zenon Rajfur, Eric Vitriol, Klaus Hahn,