Efficiency of some types of bacteria on producing biofuels from wastes of writing paper

The study included isolation and diagnosis of some types of anaerobic and aerobic bacteria capable of decomposition and fermentation of waste papers cellulose. Forty samples were collected from different environments (agricultural soil, wastewater, and normal soil) and three types of bacteria were diagnosis and isolated (Clostridium phytofermentous, Eschrichia coli, and Pseudomonas aeruginosa). The ability of studied bacteria on the production of cellulose decomposing enzymes was evaluated using UV absorbance method. Highest activity was obtained via Clostridium phytofermentous bacteria with maximum value of 2.52 IU/mL, while Pseudomonas aeroginosa gave 2.21 IU/mL and Eschrichia coli unit gave 2.09 IU/ml. The results showed the effect of some environmental conditions on the effectiveness of the production of the enzyme cellulose. Optimum temperature range was 35–40 °C and pH ranges of 7–9. Ethyl alcohol concentration was measured using titration method, maximum concentration of alcohol produced by Eschrichia coli, which reached 8.28 g/L, while in case of Pseudomonas aeroginosa reached 7.86 g/l, and Clostridium phytofermentous reached 6.62 g/L.