Article ID Journal Published Year Pages File Type
223538 Journal of Food Engineering 2013 8 Pages PDF
Abstract

A novel process for continuous preparation of casein phosphopeptides (CPPs) was developed using an enzymatic membrane reactor (EMR) and anion-exchange chromatography (AEC). Alcalase was selected to hydrolyse casein (with an initial concentration of 6% (w/w)) at pH 8.5 and temperature 50 °C in an enzymatic membrane reactor. CPPs were then separated from the enzymatic hydrolysates through a weakly basic macroporous anion-exchanger. The yields of CPPs and phosphorus from casein were 8.11%, 15.66% and 21.41%, and 19.10%, 52.70% and 90.01% for EMR fitted with 3 kDa, 5 kDa and 10 kDa membranes, respectively. The yield and quality of CPPs improved with increase in the nominal molecular weight cut-off (NMWCO) of the membrane. However, use of a high NMWCO membrane resulted in substantial enzyme leakage in the permeate.

► Novel continuous process for casein phosphopeptides preparation. ► Enzymatic membrane reactor combined with anion-exchange chromatography. ► Low cost and easy to industrialize technology.

Related Topics
Physical Sciences and Engineering Chemical Engineering Chemical Engineering (General)
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