| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 227122 | Journal of Industrial and Engineering Chemistry | 2015 | 4 Pages |
Abstract
Glycosyltransferase (GT) can convert flavonoids, alkaloids, and terpenoids into glycosylated forms. An oleandomycin glycosyltransferase, “OleD” GT from Streptomyces antibioticus was exploited for the glycosylation of amentoflavone. The OleD was functionally expressed in Escherichia coli BL21 (DE3) and purified enzyme was employed as the biocatalyst for the glycosylation of amentoflavone via the transfer of glucose moiety from UDP (uridine diphosphate)-glucose. As a result, amentoflavone monoglycoside was evident in HPLC and LC–ESI-MS spectrometry with an exact mass of 701. In addition, the reaction conditions were optimized with the highest conversion up to 85% at 30 min.
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Physical Sciences and Engineering
Chemical Engineering
Chemical Engineering (General)
Authors
Amit Kumar Chaudhary, In Yeub Hwang, Yoon Ju Jo, Sung Hee Choi, Eun Yeol Lee,