The possible mechanisms involved in nanoparticles biosynthesis

Several aspects of silver nanoparticle (NP) biosynthesis using Fusarium oxysporum under different conditions, that have not been previously considered, were explored. NP synthesis with different nitrate concentrations and silver sulfate instead of silver nitrate was investigated. The ability of F. oxysporum to synthesize NP under oxygen-limited conditions was also evaluated. In the presence of a nitrate reductase inhibitor, different mechanism rather than the reduction of metal ions by a nitrate-dependent reductase, was revealed. SDS-PAGE analysis indicated that a protein with 14 kDa molecular weight could be involved in a specific response of the fungus in the presence of silver ions.