Sequential optimization strategy for hyaluronic acid extraction from eggshell and its partial characterization

The optimization of hyaluronic acid (HA) extraction from eggshell was studied by using a sequential experimentation approach. Using the results of factorial experiments, optimization experiments were carried out by response surface methodology. The experimental data as well as the validation tests showed the optimum performance when the extraction pH and time were bounded in the ranges of 3.4–3.5 and 3–3.4 days, respectively, at 9 °C. The purified HA was characterized by FTIR spectrum, harmonic mean size and average molecular weight. The study depicted the potential of eggshell as an alternative source for HA extraction to use in industry and medicine.