Article ID Journal Published Year Pages File Type
229666 Journal of Saudi Chemical Society 2011 9 Pages PDF
Abstract

A high-throughout bioanalytical method based on a solid-phase extraction (SPE), and rapid liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis has been developed and validated for the quantification of mirtazapine (MRT) and desmethyl mirtazapine (DMRT) in heparinized human plasma. Plasma samples, without a drying and reconstitution step, were extracted by a simple SPE. The analytes and imipramine (internal standard, IS) chromatographed on a Betasil-C18 column. The total chromatographic run time was 1.8 min per sample. Response was a linear function of concentration in the range 0.1–100.0 ng/ml, with correlation coefficient ⩾0.9994. The assay has excellent characteristics and has been successfully applied to bioequivalence study samples for estimation of MRT and DMRT in healthy human subjects.

Related Topics
Physical Sciences and Engineering Chemical Engineering Chemical Engineering (General)
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