Compressed n-propane extraction of lipids and bioactive compounds from Perilla (Perilla frutescens)

•Extraction of Perilla frutescens oil by compressed n-propane in comparison with the classical Soxhlet methodology.•Mathematical modeling of the extraction process by the Sovová model.•All extraction conditions by compressed n-propane showed good extraction yields.•The oils extracted by compressed n-propane had higher levels of bioactive compounds and oxidative stability than classical Soxhlet methodology.

An experimental 22 design with center point in three replicates was used to investigate the influential factors, such as pressure and temperature, in the extraction of oil from Perilla frutescens using compressed n-propane as a solvent, and to compare with the classical Soxhlet method. The experiments were performed in the temperature range of 40–80 °C and pressure of 8–16 MPa at a constant flow rate of 1.0 cm3/min n-propane. All the conditions applied in the extraction using compressed n-propane provided good results and similar yields. However, the highest yield of extraction was obtained by the Soxhlet classical methodology. Moreover, higher induction times and concentrations of tocopherol and phytosterol were determined in oils resulting from compressed n-propane extraction. The Sovová mathematical model indicated a good fit for all conditions investigated.

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