| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 236948 | Powder Technology | 2013 | 6 Pages |
We aimed to investigate the stability and particle properties of antibody by means of spray drying process. A Plackett–Burman factorial design was applied to identify the main factors of formulation and process which significantly influenced the responses. The formulations were consisting of the antibody as well as different ratios of either mannitol or trehalose as stabilizer. The spray drying parameters were inlet temperature, air flow rate, solution concentration, liquid feed rate and aspiration percent. Spray drying yield, particle size, size distribution, moisture content and protein stability were evaluated as the responses. Size exclusion chromatography as well as Fourier transform infrared (FTIR) spectroscopy was used to investigate remained antibody monomer and secondary structure, respectively. Particle size distribution was between 2.48 and 10.2 μm by narrow distribution of 0.71 to 1.96. The results of this study indicate that atomization flow rate and type of sugar with various ratios could efficiently enhance particle properties.
Graphical abstractPlackett–Burman factorial design was applied to identify the magnitude and direction of the main factors of spray drying on particle and protein properties. Atomization flow rate and sugar type with various ratios could efficiently enhance particle properties including size and moisture content as well as spray drying yield and antibody stability.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Study the stability and particle properties of antibody with spray drying process. ► Application of Plackett–Burman factorial design to identify the main factors. ► Flow rate, type and ratios of sugars were main factors that affected the responses.
