| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 238291 | Powder Technology | 2009 | 6 Pages |
We have modelled the nucleation of the orthorhombic crystal form of lysozyme and subsequent crystal growth from concentration profiles established during the measurement of equilibrium in concentrated solutions of ammonium sulphate. A BCF mechanism for the crystal growth has been assumed. The second osmotic virial coefficient is used to calculate the activity coefficient of the protein in solution. A steep decrease of B22 is predicted for salt molalities higher than 6.8 m.
Graphical abstractWe have modelled the nucleation of the orthorhombic crystal form of lysozyme and subsequent crystal growth from concentration profiles established during the measurement of equilibrium in concentrated solutions of ammonium sulfate. The second osmotic virial coefficient is used to calculate the activity coefficient of the protein in solution. A steep decrease is predicted for salt molalities higher than 6.8 m.Figure optionsDownload full-size imageDownload as PowerPoint slide
