Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2402197 | Vaccine | 2015 | 8 Pages |
Abstract
To overcome the limiting antigenic repertoire of protein sub-units and the side effects of adjuvants applied in second generation vaccines, the present work combined in vitro and in vivo manipulations to develop biomaterials allowing natural antigen-loading and presentation in vitro and further activation of the immune response in vivo. 3-dimensional laser micro-textured implantable Si-scaffolds supported mouse macrophage adherence, allowed natural seeding with human serum albumin (antigen) and specific antibody and inflammatory cytokine production in vitro. Implantation of Si-scaffolds loaded with antigen-activated macrophages induced an inflammatory reaction along with antigen-specific antibody production in vivo, which could be detected even 30 days post implantation. Analysis of implant histology using scanning electron microscopy showed that Si-scaffolds could be stable for a 6-month period. Such technology leads to personalized implantable vaccines, opening novel areas of research and treatment.
Keywords
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Immunology
Authors
Ioanna Zerva, Chara Simitzi, Alexandra Siakouli-Galanopoulou, Anthi Ranella, Emmanuel Stratakis, Costas Fotakis, Irene Athanassakis,