Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2404056 | Vaccine | 2010 | 6 Pages |
Abstract
Since HLA-restricted cytotoxic T-cell responses select specific polymorphisms in HIV-1 sequences and HLA diversity is relatively static in human populations, we investigated the use of peptide epitopes based on sites of HLA-associated adaptation in HIV-1 sequences to stimulate and detect T-cell responses ex vivo. These “HLA-optimised” peptides captured more HIV-1 Nef-specific responses compared with overlapping peptides of a single consensus sequence, in interferon-γ enzyme linked immunospot assays. Sites of immune selection can reveal more immunogenic epitopes in HLA-diverse populations and offer insights into the nature of HLA-epitope targeting, which could be applied in vaccine design.
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Immunology
Authors
Coral-Ann M. Almeida, Steven G. Roberts, Rebecca Laird, Elizabeth McKinnon, Imran Ahmad, Niamh M. Keane, Abha Chopra, Carl Kadie, David Heckerman, Simon Mallal, Mina John,