Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2404246 | Vaccine | 2009 | 11 Pages |
Abstract
We designed and tested three PilA-derived vaccine candidates in a chinchilla model of ascending nontypeable Haemophilus influenzae (NTHI)-induced otitis media (OM). Delivery of antiserum directed against each immunogen conferred varying degrees of protection. Presentation of a B-cell epitope derived from the OMP P5 adhesin at the N-terminus of recombinant soluble PilA protein (as opposed to the C-terminus), resulted in a protective chimeric immunogen that combined epitopes from two distinct NTHI adhesins (type IV pili and OMP P5). Incorporating protective epitopes derived from two NTHI adhesins/virulence determinants into a single pediatric vaccine candidate to prevent OM has multiple potential inherent advantages.
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Immunology
Authors
Laura A. Novotny, Leanne D. Adams, D. Richard Kang, Gregory J. Wiet, Xueya Cai, Sanjay Sethi, Timothy F. Murphy, Lauren O. Bakaletz,