Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2404725 | Vaccine | 2010 | 6 Pages |
Abstract
Mycobacterial DNA-binding protein 1 (MDP1) is a major protein antigen in mycobacteria and induces protective immunity against Mycobacterium tuberculosis infection in mice. In this study we determined murine T-cell epitopes on MDP1 with MDP1 DNA immunization in mice. We analyzed interferon-γ production from the MDP1 DNA-immune splenocytes in response to 20-mer overlapping peptides covering MDP1 protein. We identified several CD4+ T-cell epitopes in three inbred mouse strains and one CD8+ T-cell epitope in C57BL/6 mice. These T-cell epitopes would be feasible for analysis of the role of MDP1-specific T-cells in protective immunity and for future vaccine design against M. tuberculosis infection.
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Authors
Daisuke Suzuki, Toshi Nagata, Ghada Eweda, Sohkichi Matsumoto, Makoto Matsumoto, Kunio Tsujimura, Yukio Koide,