Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2404890 | Vaccine | 2009 | 9 Pages |
Abstract
Vaccination with recombinant adenylate cyclase of Bordetella pertussis (CyaA) carrying antigen is a promising approach to target antigen-presenting cells. We have used Trogocytosis Analysis Protocol (TRAP) assays to monitor immune responses raised by different vaccination regimens with recombinant CyaA carrying the ovalbumin antigen. We find that the intradermal, intramuscular or subcutaneous routes are all superior to intravenous injections, and actually lead to a sufficiently high frequency of reactive CTL to be detected and characterized directly ex vivo by TRAP assay or other standard assays. Finally, for all routes, we find a clear boosting effect upon re-injection of the vaccine.
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Authors
Sandrine Daubeuf, Xavier Préville, Marie Momot, Yolande Misseri, Etienne Joly, Denis Hudrisier,