Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2405152 | Vaccine | 2009 | 9 Pages |
Abstract
To determine the immunogenicity and protective efficacy of the Mycobacterium tuberculosis 10 kD culture filtrate protein (CFP10), and to evaluate strategies that enhance local immunity, we used C57Bl/6 DR4 mice that were transgenic for human HLA DRB1*0401, because CFP10 contains epitopes for DRB1*0401 but not for C57Bl/6 mice. Intramuscular immunization with a DNA vaccine encoding CFP10 elicited production of IFN-γ by systemic CD4+ T cells, and one intravenous dose of the CFP10-based DNA vaccine coated with polyethylenimine (PEI) stimulated IFN-γ production by lung CD4+ cells and reduced the pulmonary bacillary burden. We conclude that CFP10 is a potential vaccine candidate and that coating vaccines with PEI enhances local protective immunity to tuberculosis.
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Authors
Peter Klucar, Peter F. Barnes, Ying Kong, Susan T. Howard, Xiuhua Pang, Fang-Fang Huang, Amy R. Tvinnereim, Buka Samten, Homayoun Shams,