Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2405899 | Vaccine | 2009 | 4 Pages |
Abstract
In this work, the propagation of the 17DD yellow fever virus in Vero cells grown on Cytodex-1 microcarriers was evaluated. After verifying that upon infection the virus adsorption step could be performed under continuous agitation, experiments were carried out in spinners and sparged lab-scale stirred-tank bioreactor to evaluate the use of a commercial serum-free medium (VP-SFM) and to investigate the effects of multiplicity of infection (MOI) and time of infection (TOI) on virus production. Virus titers as high as 8.4 × 108 pfu/mL were obtained upon infection with MOI of 0.02 and TOI of 3 days, using the serum-free medium in the sparged bioreactor.
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Authors
Marta Cristina O. Souza, Marcos S. Freire, Erica A. Schulze, Luciane P. Gaspar, Leda R. Castilho,