Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2405907 | Vaccine | 2009 | 4 Pages |
Abstract
Since Vero cells are currently considered as an acceptable cell substrate to produce a wide range of viruses, we developed a virus production platform using Vero cells adapted to grow in suspension in serum-free media. After adapting anchorage-dependent Vero cells to grow as a free-cell suspension, vesicular stomatitis virus, herpes simplex virus 1 and polio virus 1 production rates were evaluated in batch cultures using spinner flasks and perfused cultures in a bioreactor. The achieved results constitute valuable information for the development of a low-cost high-productivity process using a suspension culture of Vero cells to produce viral vaccines.
Keywords
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Immunology and Microbiology
Immunology
Authors
Cristian Paillet, Guillermina Forno, Ricardo Kratje, Marina Etcheverrigaray,