Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2406174 | Vaccine | 2010 | 12 Pages |
A nasal adenovirus-based vaccine is under development. To determine if aggregation occurs during vaccination, infectious titer (limiting dilution) and capsid integrity (dynamic light scattering) were assessed after extrusion of a model vector from two intranasal delivery devices. Preparations of 2.5 × 1012 and 1.25 × 1011 virus particles (vp)/ml were studied. Virus aggregated (∼10%) in the multi-dose vessel. Virus titer dropped by one log. Virus in the unit-dose device aggregated (∼1%). Titer remained unchanged. Aggregation was concentration dependent. Formulations prevented aggregation during actuation, freeze-thaw and long-term storage. The device, formulation and dose may significantly influence aggregation and potency of any nasal adenovirus 5-based vaccine.