Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2406276 | Vaccine | 2009 | 5 Pages |
Abstract
We report here the generation of a full-length, highly glycosylated HER-2 oncoprotein using yeast strain, Pichia Pastoris. Upon treatment of secreted HER-2 with alpha-mannosidase, reactivity with the monoclonal antibody Herceptin is significantly increased. This phenomenon is due to glycosylation via mannose of the full-length HER-2 protein that extends over the antigenic epitope, which is recognized by Herceptin. The extensive glycosylation of HER-2 in Pichia Pastoris significantly increases its recognition and uptake by dendritic cells, which could be associated with increased vaccine performance.
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Authors
Spiros Vlahopoulos, Angelo D. Gritzapis, Sonia A. Perez, Nike Cacoullos, Michail Papamichail, Constantine N. Baxevanis,