| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2406574 | Vaccine | 2007 | 7 Pages |
Abstract
The complete genome of the varicella-zoster virus (VZV) Oka vaccine strain (vOka) has been cloned into a bacterial artificial chromosome (BAC), and several BAC clones with the vOka genome have been obtained. Infectious viruses were reconstituted in MRC-5 cells transfected with the vOka-BAC DNA clones. The clones were distributed into two groups based on differences in amino acids found in ORF 62/71 region among the vOka-BAC clones. The recombinant vOka (rvOka) grew slower than recombinant Oka parental virus (rpOka), pOka and vOka. This is the first report that the vOka genome was cloned into BAC vector. The rvOka-BAC system would be useful as a vector for construction of recombinant live vaccines.
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Authors
Hironori Yoshii, Pranee Somboonthum, Michiaki Takahashi, Koichi Yamanishi, Yasuko Mori,