Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2407067 | Vaccine | 2008 | 10 Pages |
We compared murine T-cell responses to synthetic lipopeptide vaccines in which the TLR2 ligand Pam2Cys was attached to co-linear CD4+ and CD8+ T-cell epitopes of ovalbumin (OVA) in a linear or branched configuration. Mice received OVA-specific transgenic CD8+ and CD4+ T-cells followed by one injection of vaccine. Although the branched lipopeptide was more potent in activating OVA-specific CD4+ and CD8+ T-cells in the primary response, both vaccines induced cytolytic T lymphocytes (CTL) that expressed perforin, granzyme A–C, and IFN-γ mRNAs and conferred long-term protection of most mice against challenge with OVA-expressing tumor cells. OVA epitope display was reduced in tumors that developed in some mice, suggesting CD8+ T-cell dependent selection.