| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2407950 | Vaccine | 2007 | 11 Pages |
Abstract
We evaluated the effectiveness of in vivo electroporation (EP) for the enhancement of immune responses induced by DNA plasmids encoding the pre-erythrocytic Plasmodium yoelii antigens PyCSP and PyHEP17 administered intramuscularly and intradermally to mice. EP resulted in a 16- and 2-fold enhancement of antibody responses to PyCSP and PyHEP17, respectively. Immunization with 5 μg of DNA via EP was equivalent to 50 μg of DNA via conventional needle, thus reducing by 10-fold the required dose to produce a given effect. Moreover, IFN-γ responses were increased by approximately 2-fold. Data demonstrate the potential of EP to enhance immune responses to DNA vaccines against infectious agents.
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Authors
Carlota Dobaño, Georg Widera, Dietmar Rabussay, Denise L. Doolan,