Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2408311 | Vaccine | 2007 | 4 Pages |
Abstract
The E7 oncoprotein from Human Papilloma Virus (HPV) is an attractive candidate for anti-cancer vaccine development. In this study, we engineered HPV16 E7 coding sequence (wild type or mutagenized sequence, E7GGG) as fusions to β-1,3-1,4-glucanase (LicKM) of Clostridium thermocellum and produced in Nicotiana benthamiana plants using a transient expression system. Target antigens were purified and evaluated in mice for their potential as prophylactic and therapeutic vaccine candidates. Both fusion proteins induced E7-specific IgG and cytotoxic T-cell responses and protected mice against challenge with E7-expressing tumor cells. Furthermore, when administered after challenge, these plant-produced antigens prevented tumor development.
Related Topics
Life Sciences
Immunology and Microbiology
Immunology
Authors
Silvia Massa, Rosella Franconi, Rossella Brandi, Antonio Muller, Vadim Mett, Vidadi Yusibov, Aldo Venuti,