Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2410418 | Vaccine | 2006 | 11 Pages |
Abstract
An inbred A/J mouse respiratory challenge model was validated for vaccine testing against Chlamydia (C.) pneumoniae and used to screen the C. pneumoniae genome for vaccine candidates by expression library immunization (ELI). Biolistic delivery of genetic vaccine constructs elicited Th2-like immunity that was associated with inefficient elimination of C. pneumoniae. Delivery by injection elicited protective Th1-like responses. Since biolistic delivery of pools of ORFs was used in first round screening, the screen presumably selected against potent immunogens. Nevertheless, it was sufficiently accurate to identify three weakly protective antigens among all putative C. pneumoniae ORFs. The results suggest ELI discovery of highly protective C. pneumoniae vaccine candidates requires tight control of the Th1 immunity elicited by the genetically delivered library of test antigens.
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Authors
Dan Li, Alexander Borovkov, Alexander Vaglenov, Chengming Wang, Teayoun Kim, Dongya Gao, Kathryn F. Sykes, Bernhard Kaltenboeck,