Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2412713 | Vaccine | 2015 | 8 Pages |
Abstract
We have previously linked the sorting signals of the lysosome-associated membrane protein-1 (LAMP-1) to HPV-16 E7 antigen, creating a chimera, Sig/E7/LAMP-1. We found that both Sig/E7/LAMP-1-containing recombinant vaccinia virus (Vac–Sig/E7/LAMP-1) and Sig/E7/LAMP-1 DNA can generate strong antitumor immunity. To determine whether combination of Sig/E7/LAMP-1 DNA and Vac–Sig/E7/LAMP-1 can further enhance immune responses, sequential vaccination with Sig/E7/LAMP-1 DNA and Vac–Sig/E7/LAMP-1 was given. We found that priming with Sig/E7/LAMP-1 DNA and boosting with Vac–Sig/E7/LAMP-1 generated the strongest E7-specific CD8+ T cell responses. Our results encourage the use of the DNA prime/vaccinia booster regimen in future clinical trials.
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Immunology
Authors
Chien-Hung Chen, Tian-Li Wang, Chien.-Fu Hung, Drew M Pardoll, T.-C Wu,