Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2428771 | Developmental & Comparative Immunology | 2016 | 12 Pages |
Abstract
The ileal Peyers patches (IPP) of newborn germfree (GF) piglets were isolated into blind loops and the piglets colonized with a defined probiotic microflora. After 5 weeks, IgA levels in the intestinal lavage (IL) of loop piglets remained at GF levels and IgM comprised â¼70% while in controls, IgA levels were elevated 5-fold and comprised â¼70% of total Igs. Loop piglets also had reduced serum IgA levels suggesting the source of serum IgA had been interrupted. The isotype profile for loop contents was intermediate between that in the IL of GF and probiotic controls. Surprisingly, colonization alone did not result in repertoire diversification in the IPP. Rather, colonization promoted pronounced proliferation of fully switched IgA+IgM- B cells in the IPP that supply early, non-diversified “natural” SIgA antibodies to the gut lumen and a primary IgA response in serum.
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Authors
John E. Butler, Kristina Santiago-Mateo, Nancy Wertz, Xiuzhu Sun, Marek Sinkora, David L. Francis,