| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2428902 | Developmental & Comparative Immunology | 2015 | 7 Pages |
•Overexpression of mda5 induced zebrafish IFN promoter activity and conferred protection against SHRV infection in vitro.•A dominant negative mda5 transgenic zebrafish was generated to assess the in vivo function of mda5.•DN-mda5 zebrafish resistance to SHRV infection is significantly reduced and leads to higher viral burden and mortality.•Recombinant mda5 confers protection against SHRV infection in DN-mda5 zebrafish.
Melanoma Differentiation-Associated protein 5 (MDA5) is a member of the retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) family, which is a cytosolic pattern recognition receptor that detects viral nucleic acids. Here we show an Mda5-dependent response to rhabdovirus infection in vivo using a dominant-negative mda5 transgenic zebrafish. Dominant-negative mda5 zebrafish embryos displayed an impaired antiviral immune response compared to wild-type counterparts that can be rescued by recombinant full-length Mda5. To our knowledge, we have generated the first dominant-negative mda5 transgenic zebrafish and demonstrated a critical role for Mda5 in the antiviral response to rhabdovirus.
