Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2429001 | Developmental & Comparative Immunology | 2015 | 10 Pages |
•The myeloid differentiation factor 88 gene (As-MyD88) was cloned from A. sinica.•As-MyD88 is vital to the immune response and dorsal–ventral axis formation.•The As-MyD88 protein was mainly located in the head and chest of A. sinica.•The expression level of As-MyD88 was high at early stage of embryo development.•The expression level of As-MyD88 was high under bacterial infection.
Myeloid differentiation factor 88 (MYD88), a key adapter protein in Toll-like receptor signaling, affects the immune response and the formation of the dorsal–ventral axis. Here, the 1555bp full-length cDNA of MyD88 from Artemia sinica (As-MyD88) was obtained. Molecular characterization revealed that the sequence includes an 1182bp open reading frame encoding a predicted protein of 393 amino acids. The predicted protein contains a death domain in the N-terminus, and box1 and 2 motifs of the TIR domain in the C-terminus. Real-time quantitative PCR, Western blotting and immunohistochemistry were used to determine the expression level, protein production and location of As-MYD88 during embryonic development and bacterial challenge. The highest expression level during embryonic development was at the 0h and 5h stages of A. sinica. As-MYD88 was remarkably upregulated after bacterial challenge. Our results suggested that As-MYD88 plays a vital role in response to bacterial challenge, and during post-diapause embryonic development of A. sinica.