| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2429378 | Developmental & Comparative Immunology | 2013 | 8 Pages |
Mycobacterium avium subspecies paratuberculosis (MAP) can cause a chronic inflammatory bowel disease, Johne’s disease (JD), in ruminant animals. This study has explored the molecular basis of resistance and susceptibility to this disease in red deer breeds previously confirmed to express polarised phenotypes by experimental infection trials and following natural infection. Monocyte-derived macrophage cultures were obtained from uninfected red deer selected for either a resistant or susceptible phenotype. Cells were infected with MAP in vitro and gene expression analysed by RNA-Seq. Transcriptome analysis revealed a more disrupted gene expression profile in macrophages from susceptible animals compared with cells from resistant animals in terms of the number of genes up- or downregulated. Highly upregulated genes were related to chemotaxis (CXCL10, CSF3, and CCL8) and type 1 interferon signalling (RSAD2, IFIT1, IFIT2, ISG12, ISG15, USP18, and HERC6). Upregulation of these genes was observed to be greater in macrophages from susceptible animals compared to cells from resistant animals in response to in vitro MAP infection. These data support the use of transcriptomic approaches to enable the identification of markers associated particularly with susceptibility to MAP infection.
► We investigate the response of cervine macrophages to MAP infection. ► Macrophages were obtained from Johne’s disease resistant (R) or susceptible (S) deer. ► Cell transcriptome profiles were examined by RNA-Seq after MAP infection in vitro. ► Among highly upregulated genes were those related to chemotaxis and IFNα/β signalling. ► Global transcriptional profiles were more disrupted in S versus R after infection.
