Article ID Journal Published Year Pages File Type
2429417 Developmental & Comparative Immunology 2012 8 Pages PDF
Abstract

The high resistance of lizards to infections indicates that anti-microbial peptides may be involved. Through the analysis of the green lizard (Anolis carolinensis) genome and the expressed sequence tag (EST) libraries 32 beta-defensin-like-peptides have been identified. The level of expression of some of these genes in different tissues has been determined by semi-quantitative RT-PCR. Gene expression and structure analysis suggest the presence of alternative splicing mechanisms, with a number of exons ranging from two to four, similar to that for beta-defensins genes in mammals. Lizard beta-defensin-like peptides present the characteristic cysteine-motif identified in mammalian and avian beta-defensins. Phylogenetic analysis indicates that some lizard beta-defensins-like peptides are related to crotamine and crotamin-like peptides of snakes and lizards suggesting that beta-defensins and venomous peptides have a common ancestor gene.

► Anolis carolinensis genome contains at least 32 beta-defensin-like genes. ► A. carolinensis beta-defensin-like proteins present the characteristics cysteine-motif. ► Gene organization indicates a variable number of exons ranging from two to four. ► Gene expression suggests the presence of alternative splicing mechanisms. ► Some lizard beta-defensin-like proteins are related to crotamine/crotamin-like peptides of snakes and lizards.

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Life Sciences Biochemistry, Genetics and Molecular Biology Developmental Biology
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