Distribution and characteristics of ABFs, cecropins, nemapores, and lysozymes in nematodes

Several groups of antimicrobial effector molecules have been identified in nematodes, but most studies have been limited to Caenorhabditis elegans and, to a lesser extent, Ascaris suum. Although these two species are not closely related, they are not representative of overall nematode diversity. This study utilized available sequence information to investigate whether four groups of antimicrobial effectors (defensin-like antibacterial factors [ABFs], cecropins, saposin domain-containing proteins, and lysozymes) are components of an archetypal nematode immune system or more narrowly restricted. Saposin domain-containing proteins (caenopores in C. elegans) and lysozymes were widely distributed and found in most taxa, but likely have digestive as well as defensive functions. ABFs were widely distributed in fewer taxa, suggesting selective loss in some lineages. In contrast, cecropins were identified in only three related species, suggesting acquisition of this effector molecule in their common ancestor.

► I investigated four groups of antimicrobial effectors in nematodes. ► This study used sequence information from many divergent nematode species. ► Nemapores and lysozymes are broadly distributed but may also function in digestion. ► Defensin-like ABFs are broadly distributed but have been lost from some lineages. ► Cecropins are only found in Ascaris and closely-related species.