Article ID Journal Published Year Pages File Type
2429864 Developmental & Comparative Immunology 2007 9 Pages PDF
Abstract

Adenosine (A) and its derivatives have important biological functions, including the inhibition of immune responses and apoptosis induction. The aim of this work was to investigate whether A and N6-cyclohexyladenosine (CHA) regulate apoptosis in leukocyte cultures from the teleost, gilthead seabream (Sparus aurata L.) by flow cytometry using a double fluorescence staining method. Head kidney leukocytes (HKLs) were cultured for 0, 24 or 48 h. The kinetics of FDA+/PI− (viable), FDA−/PI− (apoptotic) and FDA−/PI+ (necrotic) leukocyte subpopulations were followed during the culture process. Apoptosis was induced by addition of resveratrol or staurosporine to the culture media and the study was validated by transmission electron microscopy. A and CHA did not induce but decreased apoptosis of seabream HKLs, in particular HK lymphocytes (L), after 24 h in culture. Results raise the question whether apoptosis is differently modulated by purine nucleosides in fish L and phagocytes and also in fish compared to mammalian cells.

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