Regional variations in the distributions of small intestinal intraepithelial lymphocytes (IELs) in outbred laboratory mice (Mus musculus domesticus) and the inbred strain of mice established from Japanese fancy mice (Mus musculus molossinus)

Previously, we reported regional variations in small intestinal IELs of mice. In this study, we examined the regional variations of IELs in outbred laboratory mice (ddY: Mus musculus domesticus) and the inbred strain of mice established from Japanese fancy mice (JF1: Mus musculus molossinus). IELs were isolated from the proximal, middle and distal parts of the small intestine and analyzed by flow cytometry. The percentages of γδ T cells and αβ T cell subset of extrathymic origin were higher in the proximal part while the percentages of αβ T cell subset(s) of thymic origin were higher in the distal part in both ddY and JF1 mice. Such trends in regional variations of IELs were almost the same as those found in the inbred strains of laboratory mice in our previous reports. This strongly suggests that these regional variations of IELs may be common phenomena in Mus musculus species.